G Protein-Coupled Receptor Genetics by Craig W. Stevens

Author:Craig W. Stevens
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ

Fig. 17GLP-1R agonists do not induce the CRE luc reporter in cultured primary neurons from line 187

6 Methods for Bioimaging Ligand Activation of GPCRs

6.1 Luciferase Whole Animal Bioimaging

Whole animal bioimaging of the mice followed the protocol previously described by Contag [24]. Mice were anesthetized with isoflurane and injected s.c. with 250 mg/kg d-luciferin (Xenogen) dissolved in DPBS without Ca2+ or Mg2+. Seven minutes after the luciferin injection, mice were placed in the IVIS Lumina (Xenogen) and imaged every 2 min for 45 min. Pseudo color bioluminescent images were superimposed onto photographic images of the mice and analyzed using the Living Image 3.0 software (Xenogen). Luciferase activities were quantified as photon per second per centimeter squared (ps−1 cm−2 sr−1).

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.